Dopamine Sulfonate Ligand-Assisted TiO2 Deposition Enabling Highly Efficient and Stable Perovskite Solar Cells.

The metal oxide electron transport layers (ETLs) with flat morphology and high electrical quality are essential to manufacture highly efficient perovskite solar cells (PSCs), in which the regulation of the metal oxide deposition process plays a crucial role. Herein, a judiciously designed dopamine sulfonate (DS) ligand-assisted deposition of titanium dioxide (TiO2) films approach is implemented based on electrostatic repulsion and steric hindrance of assembled ligands to improve colloidal nanoparticles dispersity in precursor and effectively inhibit their aggregation, which could enable obtaining smooth topography of TiO2 films and initiating growth of top high-quality perovskite films. Furthermore, sulfonate bridges bonded on the perovskite buried layer that is beneficial to form better buried interface contact and accelerate electron extraction. As a result, the PSCs employing DS/TiO2 ETLs exhibit the best power conversion efficiency of 24.53% with impressive storage stability and operation stability, i.e., remaining more than 88% of their initial efficiency upon storage N2 glovebox without encapsulation over 4000 h, and the efficiency does not attenuate significantly under maximum power point for 60 h.

PDZK1 suppresses TNBC development and sensitizes TNBC cells to erlotinib via the EGFR pathway.

Epidermal growth factor receptor (EGFR)-targeted drugs (erlotinib, etc.) are used to treat multiple types of tumours. EGFR is highly expressed in most triple-negative breast cancer (TNBC) patients. However, only a small proportion of TNBC patients benefit from EGFR-targeted drugs in clinical trials, and the resistance mechanism is unclear. Here, we found that PDZ domain containing 1 (PDZK1) is downregulated in erlotinib-resistant TNBC cells, suggesting that PDZK1 downregulation is related to erlotinib resistance in TNBC. PDZK1 binds to EGFR. Through this interaction, PDZK1 promotes EGFR degradation by enhancing the binding of EGFR to c-Cbl and inhibits EGFR phosphorylation by hindering EGFR dimerisation. We also found that PDZK1 is specifically downregulated in TNBC tissues and correlated with a poor prognosis in TNBC patients. In vitro and in vivo functional assays showed that PDZK1 suppressed TNBC development. Restoration of EGFR expression or kinase inhibitor treatment reversed the degree of cell malignancy induced by PDZK1 overexpression or knockdown, respectively. PDZK1 overexpression sensitised TNBC cells to erlotinib both in vitro and in vivo. In conclusion, PDZK1 is a significant prognostic factor for TNBC and a potential molecular therapeutic target for reversing erlotinib resistance in TNBC cells.

Laser Derived Electron Transport Layers with Embedded p-n Heterointerfaces Enabling Planar Perovskite Solar Cells with Efficiency over 25.

Electron transport layers (ETLs) with pronounced electron conducting capability are essential for high performance planar perovskite photovoltaics, with the great challenge being that the most widely used metal oxide ETLs unfortunately have intrinsically low carrier mobility. Herein is demonstrated that by simply addressing the carrier loss at particle boundaries of TiO2 ETLs, through embedding in ETL p-n heterointerfaces, the electron mobility of the ETLs can be boosted by three orders of magnitude. Such embedding is encouragingly favorable for both inhibiting the formation of rutile phase TiO2 in ETL, and initiating the growth of high-quality perovskite films with less defect states. By virtue of these merits, creation of formamidinium lead iodide perovskite solar cells (PSCs) with a champion efficiency of 25.05% is achieved, setting a new benchmark for planar PSCs employing TiO2 ETLs. Unencapsulated PSCs deliver much-improved environmental stability, i.e., more than 80% of their initial efficiency after 9000 h of air storage under RH of 40%, and over 90% of their initial efficiency at maximum power point under continuous illumination for 500 h. Further work exploring other p-type nanocrystals for embedding warrants the proposed strategy as a universal alternative for addressing the low-carrier mobility of metal oxide based ETLs.

Construction of ZnIn2 S4 /CdS/PdS S-Scheme Heterostructure for Efficient Photocatalytic H2 Production.

It is facing a tremendous challenge to develop the desirable hybrids for photocatalytic H2 generation by integrating the advantages of a single semiconductor. Herein, an all-sulfide ZnIn2 S4 /CdS/PdS heterojunction is constructed for the first time, where CdS and PdS nanoparticles anchor in the spaces of ZnIn2 S4 micro-flowers due to the confinement effects. The morphology engineering can guarantee rapid charge transfer owing to the short carrier migration distances and the luxuriant reactive sites provided by ZnIn2 S4 . The S-scheme mechanism between ZnIn2 S4 and CdS assisted by PdS cocatalyst is testified by in situ irradiated X-ray photoelectron spectroscopy and electron paramagnetic resonance (EPR), where the electrons and holes move in reverse driven by work function difference and built-in electric field at the interfaces. The optimal ZnIn2 S4 /CdS/PdS performs a glaring photocatalytic activity of 191.9 µmol h-1 (10 mg of catalyst), and the largest AQE (apparent quantum efficiency) can reach a high value of 26.26%. This work may afford progressive tactics to design multifunctional photocatalysts.

Potent targeted activator of cell kill molecules eliminate cells expressing HIV-1.

Antiretroviral therapy inhibits HIV-1 replication but is not curative due to establishment of a persistent reservoir after virus integration into the host genome. Reservoir reduction is therefore an important HIV-1 cure strategy. Some HIV-1 nonnucleoside reverse transcriptase inhibitors induce HIV-1 selective cytotoxicity in vitro but require concentrations far exceeding approved dosages. Focusing on this secondary activity, we found bifunctional compounds with HIV-1-infected cell kill potency at clinically achievable concentrations. These targeted activator of cell kill (TACK) molecules bind the reverse transcriptase-p66 domain of monomeric Gag-Pol and act as allosteric modulators to accelerate dimerization, resulting in HIV-1+ cell death through premature intracellular viral protease activation. TACK molecules retain potent antiviral activity and selectively eliminate infected CD4+ T cells isolated from people living with HIV-1, supporting an immune-independent clearance strategy.

Preparation and properties of a decarbonized coal gasification slag-fly ash filling material.

To promote the widespread use of fly ash (FA) and coal gasification slag (CGS) in mine filling, reducing the amount of cement and promoting the sustainable development of mining enterprises are essential. In this study, decarbonized CGS (DCGS) was prepared from CGS through decarbonization. A new DCGS-FA filling material was prepared using DCGS, FA, cement (3 wt.%), sodium sulfate (SS), and aeolian sand (AS). The effects of different mass ratios (1/9-5/5) of DCGS/FA on the properties of new filling materials were investigated. The results indicate that CGS can be used with FA to prepare filling materials after decarbonization. The flow performance of the DCGS-FA filling material is positively correlated with the mass ratio of DCGS/FA, while the mechanical properties are negatively correlated. The 28-day unconfined uniaxial compressive strength (UCS) of all specimens met the mechanical requirements (UCS ≥ 1.0 MPa). The types of hydration products were determined through X-ray diffraction, scanning electron microscopy, and energy-dispersive spectroscopy. The main hydration products of DCGS-FA filling materials are ettringite (AFt) and C-S-H gel. The results of the TG/DTG test of 28 days revealed that an increase in the DCGS/FA mass ratio would reduce the content of hydration products in filling materials. When the mass ratio increased from 1/9 to 5/5, the content of hydration products in the filling material decreased by 54.5%. This study provides a new concept for the resource utilization of CGS and FA in mine filling, which can significantly reduce the amount of cement in filling materials and promote the sustainable development of mine filling.

The Experimental Investigation on Mechanics and Damage Characteristics of the Aeolian Sand Paste-like Backfill Materials Based on Acoustic Emission.

With the wide application of the filling mining method, it is necessary to consider the influence of rock activity on the filling body, reflected in the laboratory, that is, the influence of loading rate. Therefore, to explore the response characteristics of loading rate on the mechanical and damage characteristics of aeolian sand paste filling body, DNS100 electronic universal testing machine and DS5-16B acoustic emission (AE) monitoring system were used to monitor the stress-strain changes and AE characteristic parameters changes of aeolian sand paste filling body during uniaxial compression, and the theoretical model of filling sample damage considering loading rate was established based on AE parameters. The experimental results show that: (1) With the increase in loading rate, the uniaxial compressive strength and elastic modulus of aeolian sand paste-like materials (ASPM) specimens are significantly improved. ASPM specimens have ductile failure characteristics, and the failure mode is unidirectional shear failure → tensile failure → bidirectional shear failure. (2) When the loading rate is low, the AE event points of ASPM specimens are more dispersed, and the large energy points are less. At high loading rates, the AE large energy events are more concentrated in the upper part, and the lower part is more distributed. (3) The proportion of the initial active stage is negatively correlated with the loading rate, and the proportion of the active stage is positively correlated with the loading rate. The total number of AE cumulative ringing decreases with the increase in loading rate. (4) Taking time as an intermediate variable, the coupling relationship between ASPM strain considering loading rate and the AE cumulative ringing count is constructed, and the damage and stress coupling model of ASPM specimen considering loading rate is further deduced. Comparing the theoretical model with the experimental results shows that the model can effectively reflect the damage evolution process of ASPM specimens during loading, especially at high loading rates. The research results have significant reference value for subsequent strength design of filling material, selection of laboratory loading rate and quality monitoring, and early warning of filling body in goaf.

GLUD1 suppresses renal tumorigenesis and development via inhibiting PI3K/Akt/mTOR pathway.

Growing cancer cells are addicted to glutamine. Glutamate dehydrogenase 1 (GLUD1) is one of key enzymes in glutamine metabolism and plays a critical role in the malignancy of diverse tumors. However, its role and molecular mechanism in clear cell renal cell carcinoma (ccRCC) development and progression remain unknown. In this study, analysis results of the GEO/TCGA/UALCAN database showed that GLUD1 level was downregulated in ccRCC tissues. Immunohistochemistry and western blotting results further validated the downregulation of GLUD1 level in ccRCC tissues. GLUD1 level was gradually decreased as ccRCC stage and grade progressed. Low GLUD1 level was associated with a shorter survival and higher IC50 value for tyrosine kinase inhibitors (TKIs) in ccRCC, reminding that GLUD1 level could predict the prognosis and TKIs sensitivity of ccRCC patients. High level of methylation in GLUD1 promoter was positively correlated with the downregulation of GLUD1 level and was negatively correlated with survival of ccRCC patients. GLUD1 overexpression suppressed RCC cell proliferation, colony formation and migration by inhibiting PI3K/Akt/mTOR pathway activation. Low GLUD1 level correlated with suppressive immune microenvironment (TIME) in ccRCC. Together, we found a novel tumor-suppressing role of GLUD1 in ccRCC which was different from that in other tumors and a new mechanism for inhibiting PI3K/Akt/mTOR activation and TIME in ccRCC. These results provide a theoretical basis for GLUD1 as a therapeutic target and prognostic marker in ccRCC.

Defected tungsten disulfide decorated CdS nanorods with covalent heterointerfaces for boosted photocatalytic H2 generation.

Owing to their intrinsic and pronounced charge carrier transport when facing the formidable challenge of inhibiting severe surface charge recombination, one-dimensional (1D) CdS nanostructures are promising for advancing high-yield hydrogen production. We herein demonstrate an efficient strategy of boosting interfacial carrier separation by heterostructuring 1D CdS with defective WS2. This process yields solid covalent interfaces for high flux carrier transfer that differ distinctively from those reported structures with physical contacts. As a nonnoble cocatalyst, WS2 can accept photogenerated electrons from CdS, and the sulfur vacancies existing at its edges can effectively trap electrons as active sites for H2 evolution. Moreover, due to its strong negative property, the H+ from the aqueous solution can gather around WS2. WS2 possesses a lower reaction barrier than CdS, which expedites the kinetic process for the reaction. The optimized sample exhibits a high photocatalytic H2 evolution rate of 183.4 µmol/h (10 mg photocatalyst), which is as far as we know among the top in the records for CdS-based photocatalysts. We believe this present work will be inspiring in addressing the interfacial charge carrier transfer by constructing covalent heterointerfaces.

A Phenotypic Screen Identifies Potent DPP9 Inhibitors Capable of Killing HIV-1 Infected Cells.

Although current antiretroviral therapy can control HIV-1 replication and prevent disease progression, it is not curative. Identifying mechanisms that can lead to eradication of persistent viral reservoirs in people living with HIV-1 (PLWH) remains an outstanding challenge to achieving cure. Utilizing a phenotypic screen, we identified a novel chemical class capable of killing HIV-1 infected peripheral blood mononuclear cells. Tool compounds ICeD-1 and ICeD-2 ("inducer of cell death-1 and 2"), optimized for potency and selectivity from screening hits, were used to deconvolute the mechanism of action using a combination of chemoproteomic, biochemical, pharmacological, and genetic approaches. We determined that these compounds function by modulating dipeptidyl peptidase 9 (DPP9) and activating the caspase recruitment domain family member 8 (CARD8) inflammasome. Efficacy of ICeD-1 and ICeD-2 was dependent on HIV-1 protease activity and synergistic with efavirenz, which promotes premature activation of HIV-1 protease at high concentrations in infected cells. This in vitro synergy lowers the efficacious cell kill concentration of efavirenz to a clinically relevant dose at concentrations of ICeD-1 or ICeD-2 that do not result in complete DPP9 inhibition. These results suggest engagement of the pyroptotic pathway as a potential approach to eliminate HIV-1 infected cells.

The Comparison of Clinical Efficacy of Minimally Invasive Tarsal Sinus Approach and L-Type Incision Approach Combined with 3D Printing Technology in Calcaneal Fracture.

Purpose: To explore the comparison of the reduction of the subtalar articular surface and other postoperative effects of the minimally invasive tarsal sinus approach and lateral L-shaped incision conventional approach for the treatment of calcaneal fracture with 3D printing technology. Methods: Patients who received surgical treatment for calcaneal fractures in the First Affiliated Hospital of Henan University of Science and Technology from June 2019 to December 2020 were collected. 3D printing equipment produced the affected side reduction heel bone fracture model and navigation template model. The tarsal sinus approach was used in the experimental group, and the lateral L-shaped incision approach was used in the control group. Patients were followed up 3 days, 1 month, 3 months, 6 months, and 12 months after the operation. Imaging indicators were measured 12 months after surgery, and scores from American Foot and Ankle Orthopaedic Society (AOFAS) and MSF were performed. Results: Operation time was 70.52 ± 13.16 in the control group and 55.24 ± 12.25 minutes in the experimental group (P < 0.001). Intraoperative blood loss was 98.77 ± 18.65 in the control group and 89.56 + 17.54 in the experimental group (P > 0.05). The duration of antibiotic use was 5.53 ± 3.24 days in the control group and 5.48 ± 4.18 days in the experimental group (P > 0.05). The frequency of fluoroscopy was 6.56 ± 1.72 in the control group and 3.88 ± 1.05 in the experimental group (P < 0.001). Fracture healing time was 3.24 ± 0.52 months in the control group and 3.08 ± 0.58 months in the experimental group (P > 0.05). The postoperative Böhler angle was 28.31 ± 3.14 in the control group and 29.24 ± 2.76 in the experimental group (P > 0.05). Postoperative subtalar articular displacement (step > 2 mm) was observed in 4 patients in the control group and 1 in the experimental group (P < 0.05). MSF score was 90.12 ± 4.85 in the control group and 91.36 ± 2.58 in the experimental group (P > 0.05). Conclusion: The study found that the experimental group was significantly better than the control group in terms of the operation time, intraoperative fluoroscopy times, and success rate of reduction of the subtalar articular surface. 3D printing technology can shorten the operation time, accurately reduce the fracture block, and reduce the secondary trauma, which is conducive to the functional recovery of the affected foot.

P5A ATPase controls ER translocation of Wnt in neuronal migration.

The Wnt family contains conserved secretory proteins required for developmental patterning and tissue homeostasis. However, how Wnt is targeted to the endoplasmic reticulum (ER) for processing and secretion remains poorly understood. Here, we report that CATP-8/P5A ATPase directs neuronal migration non-cell autonomously in Caenorhabditis elegans by regulating EGL-20/Wnt biogenesis. CATP-8 likely functions as a translocase to translocate nascent EGL-20/Wnt polypeptide into the ER by interacting with the highly hydrophobic core region of EGL-20 signal sequence. Such regulation of Wnt biogenesis by P5A ATPase is common in C. elegans and conserved in human cells. These findings describe the physiological roles of P5A ATPase in neural development and identify Wnt proteins as direct substrates of P5A ATPase for ER translocation.

SDHB Suppresses the Tumorigenesis and Development of ccRCC by Inhibiting Glycolysis.

Metabolic reprogramming is the prominent feature of clear cell renal cell carcinoma (ccRCC). Succinate dehydrogenase subunit B (SDHB) is one of subunits of mitochondrial respiratory chain complex II. The loss of SDHB function is closely related with metabolic changes in kidney cancer cells. However, the role and molecular mechanism of SDHB in ccRCC occurrence and progression are still unclear. In this study, the results of bioinformatics analyses on GEO, TCGA and oncomine databases and immunohistochemistry showed that the expression level of SDHB was downregulated in ccRCC tissues. SDHB level was gradually downregulated as ccRCC stage and grade progressed. The low level of SDHB was associated with poor prognosis of ccRCC patients, especially for advanced ccRCC patients. Increased methylation levels in SDHB gene promoter led to the downregulation of SDHB level in ccRCC tissues. SDHB was correlated with many metabolism related genes and its interacting proteins were enriched in metabolic pathways. SDHB overexpression suppressed the proliferation, colony formation and migration of ccRCC cells by inhibiting aerobic glycolysis. SDHB may be a potential prognostic marker and therapeutic target for ccRCC.

The antioxidant capacity evaluation of polysaccharide hydrolyzates from pumpkin using Caenorhabditis elegans model.

Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design. The aim of this study was to explore the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans. In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively. Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05). Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05). Moreover, the antioxidant effect of PPe-S was superior to PPe at the concentration of 4.0 mg/ml. In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents. PRACTICAL APPLICATIONS: OS plays an important role in the pathogenesis of metabolic diseases, including type 2 diabetes. It is widely acknowledged that diabetes and its complications pose a threat to human's health, and the number of people with diabetes will expand to 640 million in the 2040 year. Current studies have shown that all diabetes drugs have a kind of side effects. Fortunately, researchers have found and confirmed that plant-derived polysaccharide had a notable hypoglycemic effect via reducing the OS level in cell and tissue, and could decrease the diabetes symptoms as well. In this study, we proved that the polysaccharide derived from pumpkin could effectively ameliorate the OS level in C. elegans, including decreasing the damage of biofilm and ROS level. Therefore, our study shows that there is a high potential for pumpkin-derived polysaccharide and its hydrolyzates to be a bioactive component to prevent diabetes. In other words, this research can be applied to diabetes prevention and other diseases induced by OS.

Periodontal Disease and Age-Related Macular Degeneration: A Meta-Analysis of 112,240 Participants.

OBJECTIVE: Epidemiological studies have shown a correlation between periodontal disease (PD) and age-related macular degeneration (AMD). However, the results have been inconsistent, and no relevant meta-analysis has been performed on this topic. Hence, we performed a meta-analysis to evaluate whether the two diseases are related. Material and Methods. The PubMed, Embase, Cochrane Library, and Web of Science databases were searched up to April 20, 2020, for related articles. Two authors independently conducted literature screening and data extraction and then used the Stata 15.1 software to calculate the relative risk (RRs) and 95% confidence intervals (CIs) to assess the association between PD and AMD. RESULTS: A total of 5 observational studies involving 112,240 participants and 5,005 AMD patients were included. The results of meta-analysis using the random-effects model showed that the incidence of AMD in PD patients was 1.35 times that of non-PD patients; the difference was statistically significant (RR = 1.35, 95%CI = 1.07-1.70, P = 0.011). Sensitivity analysis showed that the results were stable. CONCLUSIONS: PD patients have a higher risk of AMD, but the causal relationship between PD and AMD has not been confirmed. Further research should be carried out to verify the exact relationship between the two.

Effects of Chlorella vulgaris polysaccharides accumulation on growth characteristics of Trachemys scripta elegans.

The present study investigated the effects of the accumulated polysaccharides in Chlorella vulgaris microalgae on the growth characteristics of Trachemys scripta elegans. Sodium alginate was used to prepare immobilized C. vulgaris, and the antioxidant effects of the accumulated polysaccharides in it were determined using Caenorhabditis elegans as a model. We determined the specific growth rates of T. s. elegans (10 in each group) and their levels of non-specific immune-related indexes (including alkaline phosphatase; total superoxide dismutase; catalase; malondialdehyde). Under optimal culturing conditions, the accumulated polysaccharide content in C. vulgaris reached 32.7% (dry weight). Polysaccharides from C. vulgaris significantly improved the hydrogen peroxide-induced oxidative stress resistance and resulted in the enhancement of stress resistance-related antioxidant enzymes, including total superoxide dismutase and catalase (p < 0.05). The accumulated polysaccharides in C. vulgaris were heteropolysaccharides comprising rhamnose, ribose, arabinose, xylose, 2-deoxy-D-glucose, mannose, glucose, galactose, and glucosamine with a molar ratio of 0.26: 0.62: 0.21: 0.10: 0.08: 0.18: 1.00: 0.42: 0.17. Compared with the control group with common feeds, suspended and immobilized C. vulgaris with higher accumulated polysaccharide levels had a positive effect on the specific growth rate of the T. s. elegans (p < 0.05). Further, the suspended and immobilized C. vulgaris with higher accumulated polysaccharide levels significantly increased serum alkaline phosphatase, total superoxide dismutase and catalase activity (p < 0.05) and decreased serum malondialdehyde levels of T. s. elegans (p < 0.05).

Evaluation of the antioxidant effects of acid hydrolysates from Auricularia auricular polysaccharides using a Caenorhabditis elegans model.

Caenorhabditis elegans is an important model organism for studying stress response mechanisms and identifying genetic pathways that influence longevity. The present study was designed to explore the in vivo-antioxidant potential and the probable mechanism of acid hydrolysates prepared from A. auricula polysaccharides (AAPHs-F) with the optimal acid hydrolysis conditions using Box-Behnken design, and C. elegans was used as a model organism. The effects of AAPHs-F on the locomotory behavior, lifespan, activities of antioxidant-related enzymes and levels of antioxidants in C. elegans were studied. In addition, the potential of AAPHs-F in up-regulating the expression of antioxidant-related genes in C. elegans, such as daf-16, skn-1, sod-1, sod-2 and sir-2.1, and the inhibition of cell apoptosis of C. elegans were also discussed. The results indicated that AAPHs-F could significantly increase the U-Turn frequency of nematodes, extend their lifespan, enhance antioxidant systems including superoxide dismutase (SOD) by 70.60%, catalase (CAT) by 73.45% and glutathione reductase (GR) by 258.68% (p < 0.01), increase the level of glutathione (GSH) by 110.22% (p < 0.01), and decrease the level of reactive oxygen species (ROS) and malondialdehyde (MDA) by 31.86% and 46.16% (p < 0.01), respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that AAPHs-F could up-regulate mRNA expression levels of daf-16, skn-1, sir, sod-1 and sod-2 in wild-type C. elegans (>1.3 fold) when treated at a concentration of 0.1 mg mL-1 (p < 0.05 or p < 0.01). AAPHs-F was concluded to be heteropolysaccharides composed of mannose, glucose and galactose with a molar ratio of 12.7 : 3.25 : 1. The molecular weight of AAPHs-F was determined to be 885.37 Da. Furthermore, AAPHs-F is mainly formed of (1 → 3)-linked-α-d-glucopyranose, and carboxyl or acetamide is present in the molecule. In summary, our studies provide evidence that AAPHs-F helps improve the antioxidant defense system, and up-regulation of stress and longevity related genes suggests the possible involvement of these genes in the prevention of stress damage in C. elegans.

Preparation of Chlorella vulgaris polysaccharides and their antioxidant activity in vitro and in vivo.

In the present study, six different polysaccharides (RFPs, MAPs, UWPs, AEPs, HWPs and CEPs) were extracted from Chlorella vulgaris using repeated freeze-thawing, microwave-assisted-, ultrasonic wave-, alkali-, hot water-, and cellulase-based methods; and antioxidant property assays were performed both in vitro and in vivo. Radical-scavenging capacity (using DPPH, superoxide and hydroxyl radicals) and metal chelating ability were assessed in vitro; Caenorhabditis elegans was used to assess antioxidant effects in vivo. Based on the in vitro screening tests, UWPs exhibited high antioxidant capacity. The UWP yield was 17.1% ±â€¯2.2%; the DPPH-, superoxide-, and hydroxyl radical-scavenging rates were 65.1% ±â€¯2.4%, 61.2% ±â€¯2.7%, and 56.2% ±â€¯2.2%, respectively, and the metal chelating ability was 63.6% ±â€¯2.5% at a concentration of 0.4 mg/mL. UWPs also exhibited high antioxidant activity in vivo. UWPs significantly increased the lifespan of C. elegans under oxidative stress induced by hydrogen peroxide compared with the control group, enhanced stress-resistance-related enzymes, including catalase and superoxide dismutase by 7.29% ±â€¯1.8% and 24.41% ±â€¯4.8%, respectively. The results of the present study indicate that the extraction methods of C. vulgaris polysaccharides were a key factor influencing antioxidant activity.

Differential inhibition of GH family 11 endo-xylanase by rice xylanase inhibitor and verification by a modified yeast two-hybrid system.

Rice xylanase inhibitor (RIXI) is a XIP-type xylanase inhibitor protein that protects rice cells from pathogenic organisms. RIXI inhibits most microbial xylanases and thus decreases their practical application. The recombinant RIXI (rePRIXI) showed evident inhibitory activities against several family 11 endo-xylanases. After interaction with rePRIXI at 50 °C for 40 min, the residual activities of reBaxA50, reBaxA, TfxA_CD214, and TfxA_CD were 55.6%, 30.3%, 30.09%, and 11.20%, respectively. Intrinsic fluorescence of reBaxA50 and TfxA_CD214 was statically quenched after interaction with rePRIXI. rePRIXI decreased hydrolysis of beechwood xylan by reBaxA50 and TfxA_CD214. Molecular dynamics simulations revealed the long loop (residues 144-153) of RIXI inserts into the catalytic cleft of family 11 xylanases. Native PAGE results revealed the formation of RIXI-xylanase complex after their interaction in the test tube. Interactions were also observed between RIXI and xylanases in living yeast cells. The results of inhibitory activity assay and modified yeast two-hybrid revealed that the inhibitory activity of RIXI on family 11 xylanase improved with the interaction strength of the RIXI-xylanase complex, indicating their positive correlation. The modified yeast two-hybrid system is relatively simple and has low cost, and its use may be extended to other studies on protein-protein interactions.

Hypoglycemic Effect of the Degraded Polysaccharides from the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Agaricomycetes).

Auricularia auricula-judae is an important culinary-medicinal mushroom. The A. auricula-judae polysaccharides (AAPs) were prepared from A. auricula-judae in the early stage through alkali extraction and deproteination with the Sevag method, and optimal acid hydrolysis conditions were established by Box-Behnken to prepare the degraded polysaccharides (AAPs-F) from AAPs. In this study, a nonenzymatic glycosylation reaction system was used for the evaluation of the inhibitory effects on the formation of advanced glycation end products (AGEs). In addition, high glucose resistance was assessed by glucose consumption of HepG2 cells and the lifespan of Caenorhabditis elegans under high sugar stress. It was found that both 0.5 mg·mL-1 AAPs and 0.2 mg·mL-1 AAPs-F could significantly inhibit AGE formation in short- and long-term glycosylation (P < .05) in a dose-dependent manner, determined by ultraviolet and fluorospectrophotometry. It indicated activity against AGE formation for different concentrations of AAPs and AAPs-F. AAPs-F at 0.5 mg·mL-1 significantly enhanced the glucose absorption of HepG2 cells by 24.4% (P < .05) in a dose-dependent manner at 24 h, and markedly extended the lifespan of C. elegans by 32.9% (P < 0.05) under high sugar stress conditions. This study demonstrated that the derived hydrolysates produced by the hydrolysis of acid had a prominent effect on the inhibition of AGE formation and relieved the stress state caused by high sugar levels.